Molecular Characterization of Mycobacterium bovis Isolates with High Copy Number of IS6110 by Restriction Fragment Length Polymorphism
نویسندگان
چکیده
Article history: Received: 27 Jan 2013 Accepted: 13 Feb 2013 Available online: 2 Nov 2013 ZJRMS 2014 Dec; 16(12):70-72 Background: Mycobacterium bovis is the causative agent of bovine tuberculosis and belongs to the Mycobacterium tuberculosis complex. M. bovis usually carries only one or a few copies of the insertion sequence IS6110 in its genome. The aim of this study was evaluation of copy number of IS6110 in M. bovis isolates by restriction fragment length polymorphism (RFLP) method. Materials and Methods: In this experimental study, 25 lymph node specimens of tuberculinpositive cattle were collected and cultured by standard methods, afterward genomic DNA was extracted by chloroform-isoamyl alcohol. Genetic studies were conducted by Pvull and DNA hybridization with IS6110. Results: Two isolates displayed more than of 4 copies of IS6110 by RFLP (IS6110-RFLP) method. Conclusion: The results of this study are unique and specific in Iran but reported in the world rarely. Therefore the new strains of M. bovis imported to Iran from other countries of the world. Copyright © 2014 Zahedan University of Medical Sciences. All rights reserved.
منابع مشابه
Combined use of Amplified Fragment Length Polymorphism and IS6110-RFLP in fingerprinting clinical isolates of Mycobacterium tuberculosis from Kerala, South India
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